Technologies
FlowCyTech
The “FlowCyTech” core facility supports your discovery process from panel design to data analysis with conventional multiparameter, Mass cytometry and innovative high-end solutions.
FLOW CYTOMETRY
Analyzers
– BD Fortessa (5 lasers; 18 fluorescence detectors)
– BD LSRII (4 lasers; 13 fluorescence detectors)
– BD FACSCanto II (3 lasers; 8 fluorescence detectors)
Cell sorting in BSL 3 conditions
– BD FACSAria (3 lasers; 8 fluorescence detectors)
Available softwares
- FlowJo 9, Tree Star
- FlowJo X, Tree Star
- Infinicyt, Cytognos
- Cytobank, Cytobank
- GemStone, Verity Software House
- SPICE, National Institute of Allergy and Infectious Diseases
Missions
- Development of flow cytometry based immune-assays
- Execution of flow cytometry-based immune assays in the context of the IDMIT center and as a service for external users
- Dissemination of knowledge related to flow-cytometry with the aim to promote harmonization and standardization between different laboratories
- Development of innovative analysis tools
- Assistance in data analysis
MASS CYTOMETRY: Cytometry by Time of Flight (CyTOF)
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Innovative mass cytometry technology was recently upgraded to the portfolio of the FlowCyTech platform. The mass cytometer (Helios Mass Cytometry, Fluidigm, USA) runs jointly by the CEA, Paris-Sud University and Bertin Pharma.
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CELL SORTING
Sorter
– BD FACSAria (3 lasers; 8 fluorescence detectors)
– FlowCyTech can assist all collaborators in data analysis using cutting edge analysis software and home-developped Batlab solution (see “Bioinformatics”)
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