Technologies

FlowCyTech

 

technologies-flowcytech

The “FlowCyTech” core facility supports your discovery process from panel design to data analysis with conventional multiparameter, Mass cytometry and innovative high-end solutions.

 

FLOW CYTOMETRY

Analyzers

- BD Fortessa (5 lasers; 18 fluorescence detectors)

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- BD LSRII (4 lasers; 13 fluorescence detectors)

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- BD FACSCanto II (3 lasers; 8 fluorescence detectors)

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Cell sorting in BSL 3 conditions

- BD FACSAria (3 lasers; 8 fluorescence detectors)

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Available softwares

  • FlowJo 9, Tree Star
  • FlowJo X, Tree Star
  • Infinicyt, Cytognos
  • Cytobank, Cytobank
  • GemStone, Verity Software House
  • SPICE, National Institute of Allergy and Infectious Diseases

Missions

  • Development of flow cytometry based immune-assays
  • Execution of flow cytometry-based immune assays in the context of the IDMIT center and as a service for external users
  • Dissemination of knowledge related to flow-cytometry with the aim to promote harmonization and standardization between different laboratories
  • Development of innovative analysis tools
  • Assistance in data analysis

 

MASS CYTOMETRY: Cytometry by Time of Flight (CyTOF)

 

Innovative mass cytometry technology was recently upgraded to the portfolio of the FlowCyTech platform. The mass cytometer (Helios Mass Cytometry, Fluidigm, USA) runs jointly by the CEA, Paris-Sud University and Bertin Pharma.

Investissements d'avenir

CELL SORTING

Sorter

– BD FACSAria (3 lasers; 8 fluorescence detectors)

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– FlowCyTech can assist all collaborators in data analysis using cutting edge analysis software and home-developped Batlab solution (see “Bioinformatics”)