Immunology & Infectiology
IDMIT has a long experience both in the immuno-monitoring of the immune system molecular responses, and in the study/evolution of viremia in the frame of drug, therapeutic or vaccine assays.
These technical skills are crucial to:
- to understand and model mechanismes of host immune responses to vaccines, drugs and therapeutics,
- to follow the infectious pathogen titers and localizations (viral reservoirs and pharmacologic sanctuaries),
- to assess immunogenicity of new therapeutic (peptides, proteins) and vaccine strategies,
- to progress in vaccine and innovative therapeutics efficacy and safety evaluation in experimental models of infection.
ELISA, ELISpots, Luminex, PCR, real-time PCR, ICS, in situ hybridization, serology, immuno-histology, immuno-phenoptyping are regularly performed. In addition, RNAsequencing and functional genomics (Next generation Sequencing: NGS) is done in collaboration with the Genome Institute (CEA-Evry, France).
Applied in fondamental, preclinical or clinical (cohorts of human biological samples) research programs, on blood, plasma, serum, body secretions (broncoalveolar secretions…), fluids (salivary, seminal fluid…), tissue homogenates, IDMITs’ Bio-assays activities allow:
- innate immune responses
- antibody measurements (IgM, IgG…)
- serum neutralization activity follow-up
- cell-mediated specific responses (CD4+, CD8+, dendritic cells…)/multiplex dosages:
- cytokines profiling (longitudinal monitoring of inflamatory markers)
- expression profiles of interferon subtypes
- pre, post-immunization, treatment, immune analyses
- immune-cells phenotypes
- pathogen (virological, bacterial, parasites) parameters:
– determination of pathogen proteins
– plasma pathogen RNA or DNA levels
– pathogen (viruses…) titers in tissue homogenates.
- Generated data are differently managed:
- centralized storage,
- long-term traceability and safety
- multi-users and projects format
- electronic lab notebook
- real-time analysis
– inter-project reports
– combined analysis of different types of data
- modelization assays via our Bioinformtic group.